High-Resolution Orbitrap Elite Mass Spectrometry
A Tool for Characterization of a Wide Range of Sample Matrices
Dr. Maryam Khaksari
Department of Chemistry
Michigan Technological University
A Thermo Scientific high-resolution Orbitrap Elite Mass Spectrometer (MS) and a Dionex UltiMate 3000 two-dimensional liquid chromatographic (2-D LC) system are present in the Chemical Advanced Resolution Methods (ChARM) laboratory. The Orbitrap Elite MS is a hybrid mass spectrometer including a dual pressure linear ion trap and a compact high-field Orbitrap. The instrument design enables high-quality, fast MSn characterization and ultrahigh resolution (R = 240,000 at m/z 400) mass measurements. The instrument along with software tools has qualitative capabilities for molecular formula identifications and structure elucidations. The combination of 2-D LC and Orbitrap Elite MS allows for targeted and non-targeted analyses for separation and qualitative quantitative mass detection of a wide range of sample analytes from environmental and atmospheric samples to metabolomic and proteomic applications. Some of the ongoing research projects in ChARM include: Targeted analysis of osmolytes in aqueous two-phase systems, targeted and non-targeted lipidomics in human blood, characterization of dissolved organic matters (DOM) in Suwannee river fulvic acid, and non-targeted analysis of toxic compounds in red maple leaf extracts.
Dr. Khaksari received her Ph.D. in Chemical Engineering at Michigan Tech in 2016. She is currently a research scientist in the ChARM lab. Her expertise is to design and develop chromatography and mass spectrometry techniques for targeted and non-targeted screening of components in complex mixtures. She manages ChARM laboratory duties including training and mentoring users, GC/MS and LC/MS method developments, sample preparations, data interpretation, optimization and validation, statistical analysis and formula assignments, laboratory safety rules and guidelines. Dr. Khaksari has presented her work in more than 15 regional, national and international conferences and is the recipient of 10 awards including the Outstanding Graduate Student Scholarship award and the Michigan Tech Portage Health Foundation Finishing Fellowship for her Ph.D. work.
Friday, November 9, 2018 at 3:00 pm to 4:00 pm
Chemical Sciences and Engineering Building, Room 101
1400 Townsend Drive, Houghton, MI 49931