Near-infrared Fluorescent Probes for Sensitive Detection of Intracellular pH Changes
Dr. Haiying Liu
Department of Chemistry
Michigan Technological University
Intracellular pH plays very important roles in many processes such as the cell functions and regulations, proliferation, cellular apoptosis, enzymatic activity, ion transport and endocytosis. Abnormal intracellular pH values are associated with many diseases such as cancer and Alzheimer’s diseases. Therefore, the accurate monitoring pH fluctuations in live cells are of significant importance to understand physiological and pathological processes, and help to explore cellular functions. Fluorescence imaging offers a powerful tool to determine intracellular pH due to its numerous advantages such as high sensitivity, rapid response, high spatial and temporal resolution, operational simplicity, noninvasiveness, and real-time monitoring. We have developed a series of near-infrared fluorescent probes based on near-infrared BODIPY, rhodamine and cyanine dyes for sensitive detection of intracellular pH changes by taking advantage of near-infrared imaging unique features such as low biological autofluorescence background, low photo bleaching issue, and deep tissue penetration. In order to overcome systematic errors of intensity-based fluorescent probes caused by the fluctuations of excitation light source, sample heterogeneity, uneven dye distribution, concentration variations, and compartmental localization, we also developed ratiometric near-infrared fluorescent probes to achieve better performance and reliability in quantitative and comparative analyses of pH changes in live cells through a built-in self-calibration function with dual emission bands.
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Dr. Haiying Liu is Professor of Chemistry at Michigan Technological University. He has developed fluorescent probes for detection of Zn(II), pH, oxygen, enzymes, reactive oxygen, nitrogen and sulfur species in live cells, and theranostics for cancer diagnosis and therapies. He has published more than 130 peer-reviewed papers in more than 30 different journals such as JACS, Angew. Chem., Chemistry of Materials, Chemical Communications and Nano Letters. His publications has received total citations of 6551 (which ranks 10 among faculties at Michigan Tech) with H-index of 46 (which ranks 6 among faculties at Michigan Tech) according to Google Scholar. He has been a PI on NIH and USDA sponsored projects, and a co-PI on NIH, USDA, NASA, NSF, Michigan 21st Century Jobs Trust Fund sponsored projects. For more detailed information of his publications, visit the link below.
Friday, November 30, 2018 at 3:00 pm to 4:00 pm
Chemical Sciences and Engineering Building, Room 101
1400 Townsend Drive, Houghton, MI 49931