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in the Chemical Advanced Resolution Methods (ChARM) Core Facility at Michigan Tech: A Tool for Characterization of a Wide Range of Sample Analytes

Environmental Engineering Graduate Seminar

Dr. Maryam Khaksari
Department of Chemistry
Michigan Technological University

Abstract: A Thermo Scientific high-resolution Orbitrap Elite Mass Spectrometer (MS) and a Dionex UltiMate 3000 two-dimensional liquid chromatographic (2-D LC) system were installed in the Chemical Advanced Resolution Methods (ChARM) Core Facility, a part of the Microanalytical Facility (MAF), at Michigan Tech. The Orbitrap Elite MS is a hybrid instrument including a dual pressure linear ion trap MS and a compact high-field Orbitrap MS. The instrument design enables high-quality, fast MSⁿ characterization with ultrahigh resolution (R = 240,000 at m/z 400) mass measurements with less than 1 ppm mass error. The instrument along with a couple of new software tools has qualitative capabilities for molecular formula identifications and structure elucidations. The combination of 2-D LC and Orbitrap Elite MS allows for separation, detection, quantitation and quantification of a wide range of sample analytes from environmental and atmospheric samples to metabolomics and proteomic applications. The facility is now available for project method development and user training. Currently, about 10 research groups at Michigan Tech are benefiting from this facility. Some of the ongoing research projects in the facility include determination of more than 20 different homologs of ethoxylated surfactants in a fresh water sample from Lake Ontario in order to reduce the number of unknown compounds in nontargeted analyses, characterization of dissolved organic matters (DOM) is Suwannee river fulvic acid samples, and identification of dissolved organic phosphorous (DOP) and their transformation products in wastewater samples.